Sun, Changbao Li, Ang Xu, Cong Ma, Jiage Wang, Huan Jiang, Zhanmei Hou, Juncai
Published in
Nutrients
Diet has a significant impact on fecal microbiota, which in turn plays an important role in human health. To evaluate the impact of dietary habits on fecal microbiota, we investigated the fecal microbial composition in vegetarians and omnivores using 16S rRNA gene sequencing, and estimated the correlation between fecal microbiota, body mass and die...
Wu, Dj Dixit, N Suzuki, E Nguyen, T Shin, Hs Davis, J Emanual Maverakis Adamopoulos, Ie
Published in
Journal of Visualized Experiments
Differentiation and activation of osteoclasts play a key role in the development of musculoskeletal diseases as these cells are primarily involved in bone resorption. Osteoclasts can be generated in vitro from monocyte/macrophage precursor cells in the presence of certain cytokines, which promote survival and differentiation. Here, both in vivo and...
Meng, X Li, W Young, F Gao, R Chalmers, L Min Zhao Song, B
Published in
Journal of Visualized Experiments
Endogenous electric fields (EFs) occur naturally in vivo and play a critical role during tissue/organ development and regeneration, including that of the central nervous system(1,2). These endogenous EFs are generated by cellular regulation of ionic transport combined with the electrical resistance of cells and tissues. It has been reported that ap...
Luxardi, G Brian Reid Fernando Ferreira Maillard, P Min Zhao
Published in
Journal of Visualized Experiments
Cells from animals, plants and single cells are enclosed by a barrier called the cell membrane that separates the cytoplasm from the outside. Cell layers such as epithelia also form a barrier that separates the inside from the outside or different compartments of multicellular organisms. A key feature of these barriers is the differential distribut...
Yang, Hsin-ya La, Thi Dinh Isseroff, R Rivkah
Published in
Journal of visualized experiments : JoVE
The physiological electric field serves specific biological functions, such as directing cell migration in embryo development, neuronal outgrowth and epithelial wound healing. Applying a direct current electric field to cultured cells in vitro induces directional cell migration, or galvanotaxis. The 2-dimensional galvanotaxis method we demonstrate ...