A Method for Comprehensive Glycosite-Mapping and Direct Quantitation of Serum Glycoproteins.
Published in Journal of proteome research
A comprehensive glycan map was constructed for the top eight abundant glycoproteins in plasma using both specific and nonspecific enzyme digestions followed by nano liquid chromatography (LC)-chip/quadrupole time-of-flight mass spectrometry (MS) analysis. Glycopeptides were identified using an in-house software tool, GPFinder. A sensitive and repro...